Manipulating Single Nano-objects with Dynamic Temperature Fields

One of the challenges of single molecule experiments in solution is the ability to localize and manipulate one or even multiple molecules. To do so a driving mechanism is required which is able to control the Brownian fluctuations of the nano-objects. Here we discuss experiments, which employ the “fuel” of Brownian fluctuations – temperature - to push nano-objects. Combined with optical feedback mechanisms effective potential landscapes can be shaped to store and manipulate single or even a well-defined number of multiple objects in a small observation volume. The paves the way for extended single molecule studies in solution or even well controlled bi- or multi molecular interaction studies.